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| Effects of ANXA2 on Biological Behaviour of Human Hepotacellular Carcinoma in Vitro |
| WEI Li-li, LI Run-ce, QIANG Rong-bing, ZHANG Nan-nan, LIU Yue-yue, ZHANG Guo-fang, YANG Mei, HOU Ying-chun |
| Lab of Tumor Cellular and Molecular Biology, College of Life Science, Shaanxi Normal University, Xi'an 710062, China |
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Abstract Objective: Investigate the correlation between ANXA2 and Hepatocellular Carcinoma (HCC) proliferation, apoptosis and motility. Method: Four siRNAs recombinants targeting to ANXA2 were introduced into SMMC-7721 cells using an optimized calcium phosphate transfection method. ANXA2 mRNA and protein levels were evaluated by using semi-quantitative RT-PCR and Western blotting. The cell proliferation, motility, and apoptosis were determined with MTT, wound healing assay, and Hoechast33258 staining methods. Results: Four siRNAs suppressed ANXA2 expression effectively with time dependence (p<0.05). Cell proliferation and motility were inhibited after ANXA2 knockdown (p<0.05), and the inhibitory efficiency was positively correlated with ANXA2 knockdown level. Conclusion: It is concluded that the over-expression of ANXA2 in HCC is closely associated with the cell malignant behaviors, and siRNA can inhibit the ANXA2 expression efficiently in SMMC-7721 cells.
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Published: 19 November 2010
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