中国生物工程杂志

The embryo of japonica cultivar Zhonghua 11 (ZH11) after flowering fifteen days was used for the callus inducing. This embryogenic callus was inoculated onto four kinds of mediums N6D，N6B5D，MsD and B5D and compared. It suggested that all of the four kinds of mediums could be used in the inducing and culturing of rice callus, but N6B5D was more appropriate than N6D, N6D and N6B5D were better than MsD, MsD was better than B5D. The N6D medium was used to the sensitivity testing of embryogenic callus to Hygromycin concertration. Embryogenic callus was sensitive to the concertration of 30 mg/L, but was thoroughly restrained from the concertration of 50 mg/L. This concertration was suggested to be used in the screening of embryogenic callus resistant to Hygromycin. Using above results, it was validated in rice genetic transformation.