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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (03): 52-55    DOI:
    
Construction of Uranium-superaccumulating Engineered E.coli
Hunan Province Key Laboratory of Pollution Control and Resources Technology, University of South China, Hengyang 421001, China
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Abstract  

PhoN gene that was amplified from Salmonella enterica serovar Typhimurium genomic DNA by PCR, was cloned into pMD18 T-Vector. Recombined transfer vector T-VectorphoN was digested by restriction enzymes of Spe I and Nde I, and then the purified phoN gene was inserted into shuttle vector pRADZ3 which was digested by the same restriction enzymes. The recombined shuttle vector pRADZ3phoN was identified by PCR and restriction analysis, and transformed into E.coli DH5α competent cell. A recombinant fusion PhoN protein was expressed in normal growth condition without induction. The expression of PhoN protein in E.coli DH5α was confirmed by Western blot. The U(Ⅵ) bioaccumulation performance of engineered E.coli was evaluated. The results showed that the maximum U(Ⅵ) bioaccumulation capacity of engineered E. coli increased four times compared to original E. coli, reaching 46.16 mg/g, and the removal rate of U(Ⅵ) was 92.32%.



Key wordsBioaccumulation      PhoN gene      Engineered strain      Uranium     
Received: 29 September 2009      Published: 25 March 2010
Cite this article:

LIANG Rong-Jun, XIE Shui-Bei, LI Shi-You, TANG Dong-Shan, LIU Ying-Jiu, LIU Jin-Xiang. Construction of Uranium-superaccumulating Engineered E.coli. China Biotechnology, 2010, 30(03): 52-55.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I03/52

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