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| Development of Chimera protein: sFcεRIα/mIg (IgG2) |
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Abstract Abstract IgE plays a key role in type I hypersensitivity reactions. The blocks of IgE binding to its high affinity receptor FcεRI can efficiently eliminate allergic reaction. The sFcεRIα/mIg（IgG2）was constructed by gene fusion of the extracellular portion of α subunit of FcεRI receptor with human IgG2 hing region, CH2 and CH3 region. The chimera could be a markedly longer plasma half-life than the soluble extracellular domain. This chimeric protein was produced by transfecting Chinese Hamster Ovary cells (CHO). Following affinity purification, the apparent molecular weight of sFcεRIα/mIg（IgG2）, which showed high affinity with human and mouse IgE， was above 170 kDa. This study provided a good foundation for industrialization of this new engineered soluble protein for type I hypersensitivity diseases.
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Received: 29 September 2009
Published: 19 October 2010
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Corresponding Authors:
Hu Pinliang
E-mail: 327hu@sina.com
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