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| Effects of RPB5-mediating Protein (RMP) on Cell Cycle of Hepatoma Cells |
| GUO Yun-lan, YANG Hui-cui, LIAN Xiao-ning, CAO Kai, WANG Xiao-ting, ZHONG Lei, WEI Wen-xiang |
| College of Basic Medicine and Biological Sciences, Soochow University, Suzhou 215123,China |
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Abstract Obiective: To study the effects and mechanisms of RMP on cell cycle of human hepatoma cells SMMC-7721.Methods:Construct the shRNA recombinant eukaryotic expression vector pGPU6-Neo -RMP. Establish shRNA RMP stable cell lines;RT-PCR was used to detect the interference efficacy against RMP gene; Flow cytometry was applied to measure the effect of RMP on cell cycle of SMMC-7721,and then the expressions of related genes of cell cycle were detected by RT-PCR.Results: The shRNA recombinant eukaryotic expression vector pGPU6-Neo- RMPi was successfully constructed. The positive monoclones were selected and stably interfered RMP cell line was obtained. Flow cytometry demonstrated that G2/M arrest was discovered in SMMC-7721-RMPi stable cell line. Conclusion: RMP could adjust the cell cycle by adjusting that the G2/M checkpoint relevance gene Cyclin B, CDK1 and P21 expressing.
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Received: 03 September 2010
Published: 27 May 2011
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