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| Study on Tolerance to Low Temperature of transgenic Aloe with TaDREB gene |
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Abstract In this experiment, plant expression carrier containing TaDREB has constructed in plasmid pBIR1. Aloe stems were used as explants for gene transformation mediated by Agrobaterium. Infected tissues were selected using G418 to generated transformants. 58 resistant plantlets were obtained from the infected explants screened by G418. The designed primers according to the selective gene nptII and the target gene of TaDREB were used for analysis all of the G418 resistant plantlets. PCR results demonstrated that TaDREB were successful transferred into aloe genomic with the transformation efficiency of 0.22%. The transgenic aloe plants were treated under 4℃ for two weeks and -20℃ for 30min.The analysis showed that the leaves of negative plants appear severe evidence of freeze injury with brown, withered and translucent, while the positive plants appear good growing condition. The isozyme patterns such as peroxidase （POD） and superoxide dismutase （SOD）of transgenic plants which are stressed 14 days under low temperature are analyzed by polyacrylamide gel electrophoresis. The results indicated that the trend of SOD and POD activities of transgenic plants are down-up-up-up, and that of non-transgenic plants are down-up-down-down. The average value of relative electrical conductivity in positive plants was 0.462 which was lower than 0.685 in the negative plants. It is supposed that transformation of this kind of gene could improve the resistant ability of aloe.
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Received: 25 May 2009
Published: 10 May 2010
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