Abstract Compared with C3 plant, C4 plant had evident growth advantage, higher rates of water and nutrition using, and higher bio-yield than C3 plant, Sugarcane was one of the typical C4 plants. DNA was extracted from sugarcane leaves, primers were designed by the cDNA sequence of PPDK gene from GenBank. Then DNA was amplified by LA-PCR(Long Acute PCR) method, ligated into pMD18-T vectors, transformed E.coli. JM109, sequenced. Full-length PPDK gene sequence of sugarcane was obtained, the sequence was 13.5Kb in length. For convenient of the next experiment, two digest sites(XhoI and NotI) were introduced into primers, the full-length PPDK gene was splitted to two parts, each ligated into pMD18-T Simple vectors, transformed E.coli. JM109, whole PPDK gene clone of sugarcane was finished, preparation of transferring it into C3 plant was made. The strains were storaged in our lab.
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