An improved staining method for two-dimensional gel electrophoresis

China Biotechnology

技术与方法

An improved staining method for two-dimensional gel electrophoresis

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Abstract An improved staining method for two dimensional gel electrophoresis was introduced in this paper. Staining the gel was composed of two steps: firstly to dye with Coomassie brilliant blue and excise protein spots in gel, secondly to perform silver re-staining. This method could increase the number of visible protein spots in gel to great extent, which could not only avoid loss of low-abundance spots in single Coomassie brilliant blue dying gel due to its lower sensitivity, but also avoid loss of high-abundance spots in single silver staining gel due to its higher sensitivity. At the same time Coomassie brilliant blue dying was compatible with silver staining perfectly so it's an effective and economical method.

Key words： Coomassie brilliant blue dye silver staining sensitivity two-dimensional gel electrophoresis

Received: 22 December 2005 Published: 25 June 2006

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Cite this article:

. An improved staining method for two-dimensional gel electrophoresis. China Biotechnology, 2006, 26(06): 71-74.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I06/71

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