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| The Study and Design Methods of Serum-Free Medium for Animal Cells |
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Abstract With the expanding scale of animal cell culture and increasing demand of biopharmaceutical, the development of serum-free medium based on cell lines and products has become a major task in the field of cell engineer. Identifying and optimizing the sera substitute and the base medium is the most important work in the study of serum-free medium. The interactions among medium components, the high intrinsic variance in cell culture assays and restriction on numbers of conditions per experiment can be studied by using statistical method. By using microarray and proteomic analysis, we can identify the receptors for growth factors, hormones and cytokines, cell adhesion molecules and other components of cell signaling pathway. Thus the additive components can be determined as regulating molecules (largely ligands) of medium. The aim of this review is to provide some idea for serum-free medium study through systematically summarizing the newly and commonly used serum-free medium study and design approaches and their characteristics.
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Received: 11 September 2006
Published: 10 May 2010
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