Lipase Extraction of Cryytococcus neoformans by Aqueous Two-phase System

China Biotechnology

2007, Vol. 27

Issue (6): 71-75 DOI:

Lipase Extraction of Cryytococcus neoformans by Aqueous Two-phase System

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Abstract

Lipase extraction of Cryytococcus neoformans using aqueous two-phase system has been studied in this paper.The polyethylene glycol(PEG)/salt system has been systematicly researched,the system of PEG/（NH4）2SO4 was better to separate this lipase.Many factors which affecting the ability of phase separation and extraction has been investigated. In this case, PEG 15%,（NH4）2SO4 22.5%, pH8.0 without NaCl was the optimized condition,and the partition coefficient and purification multiple was 6.8 and 7.5 respectively.Its specific activity got to 40.3 U/mg protein.

Key words： Lipase Cryytococcus neoformans Aqueous two-phase System Extraction

Received: 24 October 2006 Published: 25 June 2007

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Cite this article:

. Lipase Extraction of Cryytococcus neoformans by Aqueous Two-phase System. China Biotechnology, 2007, 27(6): 71-75.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I6/71

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