Abstract CX-conjugated antigen(BSA-CX) were produced by by methods of EDC.The spleen cells of BALB/C mouse immunized by BSA-CX were fused with SP2/0 plasmacytoma cells using PEG4000,and selected cultured with HAT and HT medium.A hybridoma cell line of 2H8-B1-F4 was secreened for specificity to CX,and cloned by limited dilution method for 3 times,which secrete high affinity and stable monoclonal antibodies against CX with indirect ELISA. The antibody was characterized by IgG1 isotype, the titers of antibody in cell supernatant and ascites were 1:1000 and 1:4×105,respectively.The mAb against CX was selective toward Dicloxacillin、Oxacillin with cross-reactivity of about 19.6％ and 21.3％,which can be used to detect CX residues by competitive ELISA.
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