Abstract Proteins extracted from two varieties of Chinese roses' leaves were separated by two- dimensional polyacrylamide gel electrophoresis (2-DE) with immobilized pH gradient (IPG). Many difference proteins were isolated with molecular weights ranging 10-30 kD and pI5-6. Three proteins of high levels observed in a gel were excised and identified using peptide mass fingerprinting and MS-MS. A summary of the identified proteins and their putative functions are presented. They are identified as eIF-5A、LEA protein and Hsp17.5. Functions of these proteins in plant tolerance to high temperature were discussed in this paper.
Received: 09 December 2005
Published: 25 April 2006
