The NS1 gene of H5N1 Avian Influenza Virus(AIV) Expressed in Insect Cells

China Biotechnology

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The NS1 gene of H5N1 Avian Influenza Virus(AIV) Expressed in Insect Cells

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Abstract The NS1 gene of H5N1 subtype of highly pathogenic influenza virus was subcloned into transfer plasmid pFastBac1, then the recombinant transfer plasmid pFastBac1- NS1 was transformed into DH10Bac competent cells. The recombinant transposition rBacmid- NS1 was obstained by screenining of white plaque and was identified by PCR. After the rBacmid- NS1 transfected into Sf9 cells, the recombinant baculovirus rBV- NS1 was harvested. The expressed NS1 protein was analyzed by SDS-PAGE and Western blot. The specific protein band of 26ku was obtained, and it can react with the sera of infected duck but can not react with that of immunized duck. So it was suggested that the NS1 protein can take as diagnosis antigen to differentiate the infected birds from immunized ones.

Key words： NS1 gene baculovirus expression differentiating antigen avian influenza virus H5N1

Received: 03 April 2006 Published: 25 June 2006

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. The NS1 gene of H5N1 Avian Influenza Virus(AIV) Expressed in Insect Cells. China Biotechnology, 2006, 26(06): 40-44.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I06/40

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