Whole genome shuffling to enhance activity of fibrinolytic enzyme-producing strains

China Biotechnology

2007, Vol. 27

Issue (10): 39-43 DOI:

Whole genome shuffling to enhance activity of fibrinolytic enzyme-producing strains

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Abstract

DC－12 is a Bacillus strain with strong fibrinolytic activity was screened from Douchi. In this thesis ,a novel breeding technology ---whole genome shuffling was applied to enhance the production of fibrinolytic enzyme. First of all ,a candidate mutant library was constructed by treating DC-12 with ultraviolet rays and HN02,respectively. Based on studying the optimum conditions for protoplast preparation and regeneration of DC-12,multi-parental whole genome shuffling with 4 strains was conceded by protoplasts electrical fusion ,then combines with the screening method of double-inactive protoplast , two strains with higher yield of fibrinolytic enzyme were obtained effectively, which can descend stably after five generations ,and their enzyme production reached 2710 IU/ml ,which was increased by 4-5 fold compared with the mutants , respectively.

Key words： genome shuffling in activate protoplastseletrofusio genome shuffling inactivate protoplasts electrical fusion

Received: 27 February 2007 Published: 25 October 2007

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Cite this article:

. Whole genome shuffling to enhance activity of fibrinolytic enzyme-producing strains. China Biotechnology, 2007, 27(10): 39-43.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I10/39

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