The investigation of the effect on human Hepatoma Cell apoptosis induced by Resibufogenin

China Biotechnology

研究报告

The investigation of the effect on human Hepatoma Cell apoptosis induced by Resibufogenin

Download:

HTML

PDF(0KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract AIM to study the mechanism of Resibufogenin inhibited on the growth of human hepatoma cell by analyzing the apoptosis induced by Resibufogenin. METHODS The apoptosis was observed by means of Flow Cytometry. The effects of Resibufogenin on DNA synthesis and expression of correlative proteins in hepatoma cell apoptosis was observed by means of Western blotting and cytochemical method. RESULT It was showed that Resibufogenin could induce Bel7402 cell (human hepatoma cell) to apoptosis. The rate of apoptosis exceeds 50 percent. The expression of inhibiting apoptosis gene bcl-2 decrease after 24 hours in the group of 1.0 μm Resibufogenin and decreased more significantly after 48、72 hours; the expression of promoting apoptosis gene Bax increased after 24 hours and increased more significantly after 48、72 hours. CONCLUSION the resμlt indicate that inducing neoplasm cell apoptosis may be one mechanism of the Resibufogenin inhibiting and killing the human Hepatoma Cell .

Key words： Flow Cytometry human hepatoma cell apoptosis Resibufogenin Western blotting

Received: 24 October 2005 Published: 25 June 2006

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

. The investigation of the effect on human Hepatoma Cell apoptosis induced by Resibufogenin. China Biotechnology, 2006, 26(06): 36-39.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I06/36

[1]	TAO Shou-song,REN Guang-ming,YIN Rong-hua,YANG Xiao-ming,MA Wen-bing,GE Zhi-qiang. Knockdown of Deubiquitinase USP13 Inhibits the Proliferation of K562 Cells[J]. China Biotechnology, 2021, 41(5): 1-7.

[2]	DUAN Yang-yang,ZHANG Feng-ting,CHENG Jiang,SHI Jin,YANG Juan,LI Hai-ning. The Effect of SIRT2 on Apoptosis and Mitochondrial Function in Parkinson’s Disease Model Cells Induced by MPP⁺[J]. China Biotechnology, 2021, 41(4): 1-8.

[3]	HE Ling-ling,LUO Ting-ting,CHANG Yan,WANG Ya-zhe,YUAN Xiao-ying,SHI Wei-hua,LAI Yue-yun,SHI Hong-xia,QIN Ya-zhen,HUANG Xiao-jun,LIU Yan-rong. Analysis on the Laboratory Examination Characteristics in 28 Patients with Acute Megakaryoblastic Leukemia[J]. China Biotechnology, 2019, 39(9): 2-10.

[4]	ZHAO Si-shu,LIU Lu,LIU Fang,QIU Hai-rong,FAN Lei,LI Jian-yong,WU Yu-jie. Diagnostic Value of CD11c Antigen in Patients with Chronic Lymphocytic Leukemia[J]. China Biotechnology, 2019, 39(9): 19-24.

[5]	YUAN Xiao-ying,WANG Ya-zhe,SHI Wei-hua,CHANG Yan,HAO Le,HE Ling-ling,SHI Hong-xia,HUANG Xiao-jun,LIU Yan-rong. Methodological Study on Flow Detection of PNH Clone and Its Clinical Screening Significance[J]. China Biotechnology, 2019, 39(9): 33-40.

[6]	HUI Yi-hua,WANG Hai-na,QI Yu-feng,CAO Xue-ling,GUAN Xue-mei,DUAN Jing-jing,DUAN Yi- jun,WANG Yan- feng,SU Wen. Normal Reference Range of Lymphocyte Subsets in Healthy Adults in Shanxi Province[J]. China Biotechnology, 2019, 39(9): 41-49.

[7]	ZHU Yongzhao,TAO Jin,REN Meng-meng,XIONG Ran,HE Ya-qin,ZHOU Yu,LU Zhen-hui,DU Yong,YANG Zhi-hong. Autophagy Protects Against Apoptosis of Human Placental Mesenchymal Stem Cells of Fetal Origin Induced by Tumor Necrosis Fator-α[J]. China Biotechnology, 2019, 39(9): 62-67.

[8]	Hang Hai-ying,Liu Chun-chun,Ren Dan-dan. Development, Application and Prospection of Flow Cytometry[J]. China Biotechnology, 2019, 39(9): 68-83.

[9]	PENG Xian-gui,YANG Wu-chen,LI Jia,GOU Yang,WANG Ping,LIU Si-heng,ZHANG Yun,LI Yi,ZHANG Xi. The Application of Related Cytomorphological Technology in Hematological Neoplasms Research Progress[J]. China Biotechnology, 2019, 39(9): 84-90.

[10]	Ye LIU,Yue PAN,Wei ZHENG,Jing HU. miR-186-5p is Expressed Highly in Ethanol-induced Cardiomyocytes and Regulates Apoptosis by Target Gene XIAP[J]. China Biotechnology, 2019, 39(5): 53-62.

[11]	Lu WANG,Li-yuan YANG,Yu-ting TANG,Yao TAO,Li LEI,Yi-pei JING,Xue-ke JIANG,Ling ZHANG. Effects of PKM2 Knockdown on Proliferation and Apoptosis of Human Leukemia Cells and Its Potential Mechanism[J]. China Biotechnology, 2019, 39(3): 13-20.

[12]	Xiang HUANG,Jie YANG,Pei-yan HE,Zhi-hui WU,Hui-lan ZENG,Xin-Ning WANG,Jian-wei JIANG. *Molecular Mechanism of Inducing 2774-C10 Cell Apoptosis and G₁/S Cell Cycle Arrest by Ethanol Extract from Elephantopus mollis* H.B.K.**[J]. China Biotechnology, 2018, 38(4): 17-23.

[13]	DAI Li-ting, WU Zhong-nan, HUANG Xiang, YANG Jie, ZENG Hui-lan, WANG Guo-cai, JIANG Jian-wei. *Molecular Mechanism of Inducing GLC-82 Cells Apoptosis by Ethanol Extract from Wedelia prostrate(Hook.et Arn.) Hemsl*[J]. China Biotechnology, 2017, 37(8): 1-7.

[14]	XU An-jian, LI Yan-meng, LI Si-wen, WU Shan-na, ZHANG Bei, HUANG Jian. The Effect of PHP14 Knockdown on Lung Cancer Cells Apoptosis and Its Mechanism[J]. China Biotechnology, 2017, 37(7): 12-17.

[15]	LI Yan-wei, MA Yi, HAN Lei, XIAO Xing, DANG Shi-ying, WEN Tao, WANG De-hua, FAN Zhi-yong. A Preliminary Study on Fas Apoptosis Inhibitory Molecule FAIM 1 Inducing and Simple Obesity[J]. China Biotechnology, 2017, 37(6): 37-42.

Viewed

Full text

Abstract

Cited

Shared

Discussed