Analyzing Role of △5 Desaturase in Biosynthesis of Arachidonic Acid Using Real time PCR

China Biotechnology

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Analyzing Role of △5 Desaturase in Biosynthesis of Arachidonic Acid Using Real time PCR

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Abstract Arachidonic acid is an essential fatty acid in human nutrition and a biogenetic precursor of the biologically active prostaglandins and leukotrienes. Δ5 desaturase catalyzes the Δ5 dehydrogenation of di-homo-γ-linolenic acid to form arachidonic acid in biosynthetic pathway of arachidonic acid. Using real time PCR technology, the transcriptional expression levels of gene encoding Δ5 desaturase in three Mortierella alpina strains M10, M6 and M23, and in different growth phase of high arachidonic acid yielding strain M6, were determined. Results showed that there was a distinct corelationship between mRNA transcript level of Δ5 desaturase gene and biosynthesis of arachidonic acid. Results indicated that Δ5 desaturase plays an important role in arachidonic acid biosynthesis.

Key words： real time PCR Mortierella alpina Δ5 desaturase Arachidonic Acid

Received: 14 March 2006 Published: 25 August 2006

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. Analyzing Role of △5 Desaturase in Biosynthesis of Arachidonic Acid Using Real time PCR. China Biotechnology, 2006, 26(08): 57-61.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I08/57

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