Expression and Bioactivity Assay of a Novel Clamworm Antimicrobial Peptide Perinerin in Pichia pastoris

China Biotechnology

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Expression and Bioactivity Assay of a Novel Clamworm Antimicrobial Peptide Perinerin in Pichia pastoris

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Abstract Abstract: To obtain large amounts of active perinerin and characterize its main physiochemical features, we expressed perinerin in Pichia pastoris. Intact Perinerin gene amplified by the gene SOEing method（Gene splicing by overlap extension）was cloned into expression vector pPICZαA and obtained recombinant vector pPICZαA-PEN, then pPICZαA-PEN was expressed in the Pichia pastoris GS115,The expressed sample was analyzed by Tricine-SDS-PAGE, Our results showed that Pichia pastoris was a suitable system producing the secreted form of Perinerin. Bioactivity assay showed that the recombinant Perinerin had marked antimicrobial effects.

Key words： Pichia pastoris Expression Antimicrobial peptide Perinerin

Received: 26 July 2006 Published: 25 December 2006

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. Expression and Bioactivity Assay of a Novel Clamworm Antimicrobial Peptide Perinerin in Pichia pastoris. China Biotechnology, 2006, 26(12): 45-49.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I12/45

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