| 研究报告 |
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| Isolation of proteins related to the dwarf mutant by two-dimensional gel electrophoresis in soybean |
| Li-Xia CHEN Ying-hui LI Zhao-yang REN Rong-xia GUANG Zhang-qiong LIU Fu-cong ZHENG Zai-bin HAO Ru-zhen CHANG Li-juan QIU |
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Abstract Dwarf is one of the major target traits in soybean breeding. In the study, two
dimensional electrophoresis (2-DE) was used to analyze the different proteins between the dwarf mutant (Dongze11) and its wild type (Dongnong42) which can be used to identified the gene related to the dwarf mutant.. By combination and optimization of various conditions, a sample preparation method of 2 - DE technique was established，which were used for further analyses. The total leaves proteins between mutant and wild soybean were compared with two-dimensional gel electrophoresis.There were 9 spots were notable detected, including 6 up-regulated proteins and 3 down-regulated proteins, This research established the two dimensional electrophoresis (2-DE) system which can be used to analyze leave. The similarity and difference of this system from the previous were discussed in the paper .
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Received: 01 February 2007
Published: 25 March 2007
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Corresponding Authors:
Li-juan QIU
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