Application of scaffolds in construction of random peptide libraries and targeted molecules selection

China Biotechnology

2009, Vol. 29

Issue (09): 0-0 DOI:

Application of scaffolds in construction of random peptide libraries and targeted molecules selection

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Abstract Nature is abundant of protein scaffolds. By selecting suitable protein scaffold, display and screening methods, the rational and constrained random peptide library(RPL) can be constructed and used for selection. Compared with the non-constrained RPL, it offered more opportunities for obtaining novel protein structures and more higher affinity ligands against the target molecules. At present, the protein scaffold constrained RPLs have been shown great potential in applications such as target selection, basic research, clinical diagnosis, medical therapy and so on. This review systematically introduced the structure bases, classification and construction of constrained RPL based on scaffolds, as well the recent great advances of application in selection against target molecules with S-S constrained scaffolds, antibodies, Zinc finger protein, Z domain, FN3 domain as important examples.

Key words： scaffold domain random peptide library constrained peptide library

Received: 28 April 2009 Published: 10 May 2010

Corresponding Authors: Guo HongGuang E-mail: hongsinb.com@163.com

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	GUO Hong-Guang
	WU Hai-Jiang
	MAO Can-Quan

Cite this article:

. Application of scaffolds in construction of random peptide libraries and targeted molecules selection. China Biotechnology, 2009, 29(09): 0-0.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I09/0

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