Screening of Strains and Fermentation for Improving the Properties of Cottonseed Meal Protein

China Biotechnology

2010, Vol. 30

Issue (10): 33-38 DOI:

Screening of Strains and Fermentation for Improving the Properties of Cottonseed Meal Protein

LIU Jun¹, ZHUGE Bin¹, FANG Hui-ying¹, ZHANG Meng², ZHUGE Jian¹

1. The Key Laboratory of Industrial Biotechnology, Ministry of Education and the Research Centre of Industrial Microbiology, Jiangnan University, Wuxi 214122,China;
2. Department of Biotechnology Durban University of Technology P.O. Box 1334 Durban 4000, South Africa

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Abstract

The content of protein is usually 38%~50% in the cottonseed meal. However, some properties of the protein make cottonseed meal unsatisfiable for the animal feed industry. To solve the problem, four predominant strains, which can efficiently degrade the macromolecular protein of cottonseed meal, were isolated from natural samples. Based on biochemical characteristic and molecular biology identification, they could be classified as B. subtilis (2 strains, marked as H1 and H7), B. cereus (1 strain, marked as H9) and A. niger (1 strain, marked as P1). Either individual strain or combined strains fermentations were carried out. The results showed that under the optimal strain combination (B. subtilis H1 and A. niger P1) fermentation of cottonseed meal, the content of acid-soluble protein increased by 25.34%, the content of small peptides increased by 11% and the content of the total soluble amino acid increased by 24%, meanwhile, the digestibility of protein increased from 44.56% to 78.61%, which were significantly higher than other strains combination. The results showed that the fermentation by strains combination (B. subtilis H1 and A. niger P1) can improve the forage utilization ratio of the cottonseed-meal-protein effectively.

Key words： Cottonseed meal Solid state fermentation Acid-soluble protein Small peptides

Received: 09 July 2010 Published: 25 October 2010

ZTFLH:

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Corresponding Authors: ZHUGE Bin E-mail: Bzhuge@yahoo.com.cn

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LIU Jun, ZHUGE Bin, FANG Hui-ying, ZHANG Meng, ZHUGE Jian. Screening of Strains and Fermentation for Improving the Properties of Cottonseed Meal Protein. China Biotechnology, 2010, 30(10): 33-38.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I10/33

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