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| Expression Profile Analysis of Rice Heat Shock Transcription Factor (HSF) Genes in Response to Plant Hormones and Abiotic Stresses |
| WAN Bing-liang, ZHA Zhong-ping, DU Xue-shu |
| Food Crop Research Institute, Hubei Academy of Agricultural Science, Wuhan 430064, China |
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Abstract Heat shock transcription factors (HSFs) are critical regulators of heat shock response of plants and participate in various plant stress responses. Rice HSFs are encoded by a multigene family but their specific functions are not well-understood. Study on expression profiles of rice HSF genes is important for illuminating the molecular mechanism by which rice respond to environmental stresses.The identification of rice HSFs genes were conducted through sequence comparing and their expression profiles in various tissues under hormone treatments and abiotic environmental stresses were also analyzed. In a genome-wide scan, twenty-five rice HSF genes were identified and called OsHsf1~ OsHsf25, respectively. The phylogenetice tree of rice and Arabidopsis HSFs was constructed through amino acid sequence alignment, twenty-five rice HSFs were classified as three groups, class A, B and C,and each group comprised one or several subgroups. Class A, B and C included thirteen, eight and four genes, respectively. The gene structure analysis showed that most rice HSF genes contain a small number of exons and introns, and are spliced into a single gene model, alternative splicing was observed only in eight members (OsHsf2, OsHsf7, OsHsf9, OsHsf11, OsHsf12, OsHsf21, OsHsf24 and OsHsf25). The expression profiles of 24 rice HSF genes were derived through quantitative PCR. Most rice HSF genes presented the tissue-specific and development-specific transcription patterns. OsHsf4, OsHsf12 and OsHsf13 showed high expression levels in all tissues analyzed, whereas OsHsf17 expressed with low transcription levels. Ten HSF genes were significantly regulated by gibberellin 3 (GA3), kinetin (KT), and abscisic acid (ABA) treatments at transcription level, among them, OsHsf1, OsHsf3, OsHsf21and OsHsf24 were down-regulated by GA3 and KT, and OsHsf1and OsHsf24 were also up-regulated by ABA. Thirteen HSF genes were observed to have a significant response to drought, salt, and heat stresses. OsHsf1, OsHsf3, OsHsf5, OsHsf11, OsHsf12, OsHsf14, OsHsf21, OsHsf23, OsHsf24, and OsHsf25 were strongly up-regulated by all three stresses. OsHsf2 and OsHsf16 were significantly induced by drought and salt stresses. OsHsf15 was up-regulated only by heat stress. Rice HSFs expression profiles, especially in response to drought, salt, and heat stresses, can provide a foundation for dissecting the functions of rice HSFs in response to abiotic environmental stresses. The HSF genes responding to abiotic stresses identified may be used to improve rice tolerance to abiotic stresses through genetic engineering technology.
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Received: 19 July 2010
Published: 25 October 2010
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| Fund: This work was supported by Hubei Agricultural Science Innovation Foundation (2007-620-001-003) |
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Corresponding Authors:
WAN Bing-liang
E-mail: ricewanbl@126.com
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