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| The Study on Fermentation Conditions of Inulase Produced by Aspergillus niger |
| CAO Ze-Gong, DONG Yu-Wei, MIAO Jing-Zhi, LV Zhao-Qi |
| Bioengineering School, Xuzhou Institute of Technology,Xu Zhou221008, China |
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Abstract The high production inulase strain was screened from the soil sample where burdock planted in Qin Village, Bayou Town, Pei County, Xuzhou. Inulase activity were determined which produced by 40 strains separated from soil. Three mold stains, C122803、D081506 and D081513, which had higher ability of producing inulase were obtained by using transparent circle method as initial screening and rocker method as rescreening. Enzyme activity of the three strains were 1.411U/ml, 1.895U/ml, 1.792U/ml,separately . Enzyme activity of D081506, 1.895U/ml, was the highest. The fermentation conditions of D081506 were studied and the optimized conditions were lappa juice 2.0%, yeast extraction 1.6%, (NH4)2SO4 0.5%， NaCl 0.5%，K2HPO4 0.5% and pH 5.0. Inulase activity of D081506 was 2.9578U/ml which increased 56.09% under the condition of 27℃,140r/min, 24h.
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Received: 10 March 2009
Published: 28 July 2009
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