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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (08): 62-67    DOI:
    
Study on the High Yield Strain of Gentamicin Producer by Protoplast Fused and Fermentor Test
 GUAN Yu-Xia, LAN Ji-Xian, YAN Guang-Xin, ZHENG Jing-Shi
College of Biological Science and Technology, Fuzhou University, Fuzhou350002,China
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Abstract  

The protoplasts of Micromonospora purpurea, the high yield strains of gentammicin producer were mutagenized by diethyl sulfate (DES) and ultraviolet radiation (UV)respectively, then fused, screened by gentamicin resistance and regenerated. The average fermentation unit 2200±U/ml could be achieved by shake flask for 10 batches. The average fermentation unit 1900±U/ml could be obtained by 5L fermentor for 7 batches. The quality of the end product conformed to CP2000, BP2000 and USP26 pharmacopoeia.



Key wordsMicromonospora purpurea;protoplast mutagenesis fusion;screened resistance;yield of fermentor     
Received: 20 January 2009      Published: 28 July 2009
ZTFLH:  Q813.2  
Cite this article:

GUAN Yu-Xia, LA Ji-Xian, YAN An-Xin, ZHENG Jing-Shi. Study on the High Yield Strain of Gentamicin Producer by Protoplast Fused and Fermentor Test. China Biotechnology, 2009, 29(08): 62-67.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I08/62

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