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| Examination DNA Vaccine-binding Proteins in the Immune Cells |
| ZHONG Yi-wei, LI Jin-yao, GENG Shuang, WANG Bin |
| State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100194, China |
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Abstract Examination DNA vaccine-binding proteins in the immune cells. Cytoplasmic proteins were extracted from splenocytes of mice and were incubated with DNA vaccine, pVAX-OVA as the experimental group, and empty vector pVAX, as the control group. The bound proteins were isolated by an agarose gel and SDS-PAGE and examined by MALDI-TOF mass spectrometry to analyze their sequences. In splenocytes, the potential proteins interacted with pVAX are Foxl2,SUV420H2 and gamma actin, and the potential proteins interacted with pVAX-OVA are IQ motif containing F4. Foxl2 may improve nuclear import of DNA vaccine, which includes nuclear localization signals (NLS), conserved DNA-binding forkhead domain and interaction with transporter proteins. The interactions between DNA and those proteins may or may not be beneficial for DNA vaccine entering nuclear before its expression.
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Received: 18 June 2010
Published: 25 October 2010
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Corresponding Authors:
WANG Bin
E-mail: bwang3@cau.edu.cn
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