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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (02): 30-37    DOI:
    
Isolation and Identification of Zn2+ Binding Proteins in Streptococcus pyogenes by IMAC and LC-MS/MS
YANG Xiao-yan, YIN Xing-feng, ZHANG Liu-hui, HE Xiang, SUN Xue-song
Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, China
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Abstract  

Streptococcus pyogenes is a gram-positive pathogen that causes a variety of human diseases. The zinc binding proteins of S. pyogenes were enriched through Immobilized-metal affinity chromatography (IMAC). The proteins were further digested in-solution and analyzed by the advanced LC-MS/MS. In total, forty-eight zinc binding proteins involved in protein translation, glucose metabolism, metal transport, oxidation and other physiological processes were identified. The data presented here are helpful in understanding of the pathological mechanisms of S. pyogenes and facilitate the discovery of potential antibacterial drug targets and vaccine candidates.



Key wordsLC-MS/MS      Streptococcus pyogenes      Zn2+ binding proteins      IMAC     
Received: 19 October 2010      Published: 18 February 2011
ZTFLH:  Q513  
Cite this article:

YANG Xiao-yan, YIN Xing-feng, ZHANG Liu-hui, HE Xiang, SUN Xue-song. Isolation and Identification of Zn2+ Binding Proteins in Streptococcus pyogenes by IMAC and LC-MS/MS. China Biotechnology, 2011, 31(02): 30-37.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I02/30

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