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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (05): 78-82    DOI:
    
Improvement of MTG Production from Streptomyces hygroscopicus by Fed-batch Fermentation
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Abstract   In Streptomyces, MTG (Microbial Transglutaminase) is secreted and activated during the emergence of aerial hyphae rather than during the vegetative growth. The secretion and activation process were stopped when spores were formed. To obtain high cell density and high yield of MTG by Streptomyces hygroscopicus, the effects of exponential feeding of glucose and the addition of nitrogen source on MTG production were investigated. The results showed that keeping a high specific growth rate (0.15h-1) in 8~16h and a relative low specific growth rate (0.10h1) during later time could obtain highcell density, the DCW could reach 36 g/L which was increased by 80% compared to that in batch fermentation mode. The MTG activity reached 5.79U/ml by adding 50 g/L soybean meal in the initial medium, and was increased by 83%.

Key wordsStreptomyces hygroscopicus;high-cell-density culture;MTG;exponential feeding;nitrogen source     
Received: 15 December 2008      Published: 06 May 2009
Cite this article:

HU Xiao-Juan- Zhang-Dong-Xu- Wang-Miao- Li-Jiang-Hua- Chen-Jian- Du-Guo-Cheng. Improvement of MTG Production from Streptomyces hygroscopicus by Fed-batch Fermentation. China Biotechnology, 2009, 29(05): 78-82.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I05/78

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