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| Study on Mutated DHDPS Gene as Selectable Marker of Transgenic Plant |
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Abstract The rodipicolinate synthase gene was cloned from Nicotiana tabacum L.cv, which encodes the key enzyme in the synthesis of lysine, and then a vitro molecular reform reliefing feedback suppression was made. Some resistant seedlings were acquired using the mutated gene as selectable marker and analog of lysine as selectable reagent, and the detection results were positive by means of PCR and Real-Time PCR, however the phenotype is somewhat abnormal.
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Received: 09 December 2008
Published: 06 May 2009
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