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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (05): 66-71    DOI:
    
Cloning and Transformation of lba gene of Glycine max into Chloroplast of Chlamydomonas reinhardtii
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Abstract  

To decrease the oxygen content in the cell is a key method to improve hydrogen production in Chlamydomonas reinhardtii. To achieve this goal, we developed a new approach by transforming the leghemoglobin gene lba, which has high affinity to oxygen, into the chloroplast of C. reinhardtii to get a low dissolved oxygen in the cell and result into improvement of H2ase activity and H2 yield. The results showed that lba was successfully transformed into the chloroplast of C. reinahrdtii strain 849 and did not affect its growth significantly. This work paved the road for further regulation of lba expression in the chloroplast to improve of hydrogen production of C. reinahrdtii.



Key wordsChlamydomonas reinhardtii;leghemoglobin;bio-hydrogen production;chloroplast transformation     
Received: 14 October 2008      Published: 06 May 2009
Corresponding Authors: WU Shuang-xiu   
Cite this article:

YAN Guang-Yu- Liu-Xiao-Lei- Wang-Quan-Chi- Tun-Shuang-Xiu. Cloning and Transformation of lba gene of Glycine max into Chloroplast of Chlamydomonas reinhardtii. China Biotechnology, 2009, 29(05): 66-71.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I05/66

[1] Deffeyes K S. Hubbert's peak: The impending World Oil shortage. Princeton,NJ: Princeton University Press, 2001.5~200 [2] Ramachandran R, Menon R K. An overview of industrial uses of hydrogen. Int J Hydrogen Energy, 1998, 23: 593~598 [3] 杨素萍, 赵春贵, 曲音波, 等. 生物产氢研究与进展. 中国生物工程杂志, 2002, 22(4):44~48 Yang S P, Zhao C G, Qu Y B, et al. Journal of Chinese Biotechnology, 2002(4):44~48 [4] 管英富,邓麦村,金美芳,等. 微藻光生物水解制氢技术. 中国生物工程杂志, 2003, 23(4):8~13 Guang Y F, Deng M C, Jin M F, et al. Journal of Chinese Biotechnology, 2003, 23(4):8~13 [5] Hahn J J,Ghirardi M L,Jacoby W A. Effect of process variables on photosynthetic algal hydrogen production. Biotechnol Prog, 2004, 20(3):989~991 [6] Zhang L,Melis A. Probing green algal hydrogen production . Phil Trans R Soc Lond B, 2002, 357:1499~1509 [7] 吴双秀,王全喜. 衣藻生物制氢的研究进展. 中国生物工程杂志, 2006, 26(10):88~92 Wu S X, Wang Q X. Journal of Chinese Biotechnology, 2006, 26(10):88~92 [8] Melis A. Photosynthetic H2 metabolism in Chlamydomonas reinahrdtii. Planta, 2007, 226(5):1075~1086 [9] Kruse O, Rupprecht J, Bader K, et al. Improved phtobiological green algal cells. Joural of Biological Chemistry, 2005, 280(40):34170~34177 [10] Kundu S, Trent J T, Hargrove M S. Plants humans and hemoglobins. TRENDS in Plant Science, 2003, 8(8):387~393 [11] 陈义烘,马三梅,等. 植物血红蛋白的发现及其功能. 生命的化学, 2006, 26(5):408~410 Cheng Y H, Ma S M, et al. Life of Chemistry, 2006, 26(5):408~410 [12] Wagner. Characteristics of Modified Leghemoglobins lsolated from Soybean (Glycine max Merr.) Root Nodules. Plant Physiol, 1994, 104:1231~1236 [13] 赵亚兰,尉亚辉,等. 豆血红蛋白的研究进展. 西北植物学报, 2000, 20(4):684~689 Zhang Y L, Wei Y H, et al. Acta Botanica BorealiOccidentalia Sinica, 2000, 20(4):684~689 [14] Suthar D H, Chattoo B B. Expression of Vitreoscilla hemoglobin enhance growth and levels of alpha-amylase in Schwanniomyces occidentialis. Applied Microbiological Biotechnology, 2006, 72 (1):94~102 [15] Walker T L, Purton S, Becker D K, et al. Microalgae as bioreactors. Plant Cell Rep, 2006, 22(6):1490~1506 [16] 韩四海,胡章立,王潮岗. 外源基因在莱茵衣藻叶绿体中的表达. 生物技术通报, 2007, 1:89~94 Hang S H, Hu Z L, Wang C G. Biotechnology Bulletin, 2007, 1:89~94 [17] Leon-Banares R, Gonzalez-Ballester D, Galvan A, et al. Transgenic microalgae as green cellfactories. Trends in Biotechnology, 2004, 22(1):45~52 [18] 王潮岗,胡章立,胡炜,等. phbB基因在莱茵衣藻中的表达与分子检测. 科学通报, 2004, 49(15):1519~1522 Wang C G, Hu Z L, Hu W, et al. Journal of Science, 2004, 49(15):1519~1522 [19] Harris E H.The Chlarnydomonas Sourcebook:a comprehensive guide to biology and laboratory use . NewYork: Academic Press, 1989. 32~34,610~614,607~608 [20] Vaistij F E, Goldschmidt-Clermont M, Wostrikoff K, et al. Stability determinants in the chloroplast psbB/T/H mRNAs in Chlamydomonas reinahrdtii. The Plant Journal, 2000, 21(5):469~482 [21] Boynton J E, Gillham N W, Harris E H, et al. Chloroplast transformation in Chlamydomonas-with-high-velocity-microprojectiles. Science, 1988, 240(4858):1534~1538 [22] Schroda M, Blocker D, Beck C F. The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas. Plant J, 2000, 21(2):121~131 [23] Frustaci J M, O'Brain M R. Characterization of a Bradyrhizobium japonicum ferrochelatase mutant and isolation of the hemH gene. Journal of Bacteriology, 1992, 174:4223~4229 [24] Santana M A, PihakaskiMaunsbach K, Sandal N, et al. Evidence that the Plant Host Synthesizes the Heme Moiety of Leghemoglobin in Root Nodules. Plant Physiol, 1998,116:1259~1269 [25] Ji L, Becana M, Sarath G,et al. Overproduction in Escherichia coli and characterization of a soybean ferric leghemoglobin reductace. Plant Physiology, 1994, 106:203~209
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