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| Establishment of Transformed Mammalian Cell Lines Stably Expressing the Rabies Virus Glycoprotein |
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Abstract The glycoprotein of rabies virus strain CVS-24 was amplified by reverse transcription-polymerase chain reaction and cloned it and its fusion gene with green fluorescent protein into eukaryotic expression vector pIRES1neo. The resultant plasmid pICG was transfected into BHK-21 cell line by liposome transfection. After screening with G418, G418-resistant BHK-21 colonies were obtained and amplified. With Western blot analysis, there appeared a reaction band between the specific antibody and the expressed protein, which indicated that the protein has been expressed. By screening with indirect ELISA, three cell clones of expression vector with high level expression were established and used for further identification, named respectively ICG1, ICG2, ICG3.
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Received: 27 August 2008
Published: 27 April 2009
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