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| Cloning and Expression Analysis of BTB/POZ Gene in Banana |
| JIA Cai-hong1, ZHANG Li-li3, JIN Zhi-qiang2, ZHOU Xue-li1, LIU Ju-hua1, ZHANG Jian-bin1, XU Bi-yu1 |
1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;
2. Haikou Experimental Station, Institute of Banana, Chinese Academy of Tropical Agricultural Science,Haikou 570101,China;
3. College of Agriculture, Hainan University, Haikou 570228, China |
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Abstract A full length cDNA named MaBTB (BR-C, ttk and bab or pox virus and zinc finger), which contained 1 080bp with an open reading frame, was isolated by SSH combined with RACE technology. Sequence analysis revealed that this gene shared high identities of 86%、85%、84%、84%、86% and 87% to those of BTB/POZ from Oryza sativa(OsBTB), Medicago truncatula(MtBTB/POZ), Arabidopsis thaliana(AtBPM4), Vitis vinifera(VvBTB), Zea mays (ZmBTB)and Picea sitchensis(PsBTB)), respectively. Phylogenetic analysis showed that MaBTB was closely related to Medicago truncatula (MtBTB) and Arabidopsis thaliana (AtBPM4).The expression feature of MaBTB was investigated by RT-PCR. The result showed that under salt, ethylene and ultraviolet radiation treatment, the expression of MaBTB gradually increased. The accumulation of MaBTB decreased and then gradually increased with cold and FOC race 4 treatment. However, the accumulation of MaBTB was not distinct variety by wounding treatment.
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Received: 28 July 2010
Published: 19 November 2010
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