| 研究报告 |
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| Transfection and Expression the EGFP Gene in the Primarily Cultured Cells from Chinese Shrimp Fenneropenaeus chinensis |
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Abstract The study tried to develop the technology related to introducing the eukaryotic expression plasmid into the primarily cultured cells from Chinese shrimp (Fenneropenaeus chinensis), one of the most commercially important aquaculture marine invertebrates in China. The primary cell cultures included the adherent or suspension cultures from the haemocytes and the cell adherent cultures from the lymphoid organ（Oka organ）and ovary of the shrimp. The methods of gene transfer tried in this paper included calcium phosphate mediated transfection, liposome mediated transfection（lipofection）and electroporation. Lipofectin could be used to introduce the plasmid into the cultured cells and to express the reporter gene, EGFP（enhanced green fluorescence protein）gene, in the haemocyte suspension cultures and the cell adherent cultures from the Oka organ and ovary. The present work is to facilitate the study of shrimp immunology and transgenic studies by developing a primary culture system of the shrimp.
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Received: 13 September 2005
Published: 25 February 2006
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