| 研究报告 |
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| High Cell Density Cultivation of Recombinant Escherichia coli for Production of Fusion Protein Staphylokinase-Hirudin |
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Abstract Recombinant fusion protein staphylokinase-hirudin has been produced by adopting the dissolved oxygen feed-back fed-batch with the high cell density cultivation strategy in E.coli BL21(DE3). After selection of the strain and the preliminary optimization by flask cultivation, the fermentation was performed with comparing the differences between defined medium and complex medium in 5L fermentor. With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.
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Received: 31 March 2006
Published: 25 September 2006
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