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| Establishment of miR-106b Transgenic Mouse |
| WANG Hai-lin, QUAN Xiong-zhi, DONG Wei, ZONG Yuan-yuan, LIU Jia-lin, QIN Chuan |
| Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Key Laboratory of Human Disease Animal Model, State Administration of Tranditional Chinese Medicine, Beijing 100021, China |
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Abstract Objective:To establish a miR-106b transgenic mouse and investigate its effect on the development of Alzheimer’s disease (AD). Methods:The expression vector of miR -106b was constructed. The transgenic mouse was produced by microinjection and genotype was detected by PCR. The expression levels of miR-106b were detected by real time RT-PCR. The protein levels of TGFBR2 were detected by Western blot. Results:Five founders of miR-106b transgenic mice were established and one high-level expression line was identified. Compared with the wild type mice, the expression of TGFBR2 was increased in miR-106b transgenic mice. Conclusion:miR-106b transgenic mouse has been established and it can be used to investigate the function of miR-106b on the development of Alzheimer’s disease.
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Received: 26 August 2010
Published: 25 December 2010
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