中国生物工程杂志

Eukaryotic translation initiation factor 5A (elF5A ) is involved in celI proliferation, senescence, development and environmental response. cDNA of Chinese rose was amplified by PCR with degeneracy primer. The special fragment with 480 bp nucleic acid encoded a deduced amino acid sequence of 159 residues was obtained and named as RceIF-5A(GenBank No. EF177192). The gene was cloned into pET32a-vector and the recombination vector was named as pET32a-eIF5A. E.coli line BL21(pET32a-eIF5A) had more obvious tolerance than E. coli BL21 (pET32a) under the high(50℃) and low（4℃）temperature. By all accounts, thermotolerance enhancing was attributed to the heterogenous expression of eIF-5A gene in host cell line.