Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2007, Vol. 27 Issue (11): 82-85    DOI:
    
Isolation and characterization of a strain Z7,capble of degrading nicotine
Li-Jue
Download: HTML   PDF(513KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

A nicotine-degrading bacterial strain Z7 was isolated from tobacco growing soil at Zhangjiajie region in Hunan Province. The morphology, physiological and biochemical characteristics of strain Z7 were studied and the results showed that characteristics of this strain were essentially consistent with Agrobacterium radiobacter/tumefaciens. This strain degraded nicotine optimally at 30℃ and initial pH 7. It was able to utilize nicotine as its sole carbon source, and it could degrade 71% of nicotine under the optimized incubation conditions for 48 h. Meanwhile,the color of culture medium turned from yellowy to green and dark green, then it turned to brown. This strain might have potential applications in tobacco industry.



Key wordsNicotine      Biodegradation      Agrobacterium radiobacter      Agrobacterium tumefaciens     
Received: 06 August 2007      Published: 25 November 2007
ZTFLH:  TS414  
Cite this article:

Li-Jue . Isolation and characterization of a strain Z7,capble of degrading nicotine. China Biotechnology, 2007, 27(11): 82-85.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2007/V27/I11/82

[1] Yu-rong ZHENG,Jun-bao JIN,Xin-nian WU,Guang-zu BAI,Qiu-yan LIU. Study on Disruptive Technology of Cellulose Biodegradation ased on Multi-data Sources[J]. China Biotechnology, 2018, 38(5): 92-103.
[2] HU Chun-Hui, XU Qing, YU Hao. Characteristics and Kinetic Study of 2-Hydroxypyridine Degradation by a Novel Bacterium Arthrobacter sp. 2PR[J]. China Biotechnology, 2017, 37(8): 31-38.
[3] ZHOU Yu-cong, XIE Qiu-jin, SONG Kai, YANG Zhao-hui, CHEN Jie, LI Ya-qian. Optimal Agrobacterium tumefaciens-Mediated Transformation Technology Applied in Gene Knockout for Trichoderma atroviride[J]. China Biotechnology, 2015, 35(12): 58-64.
[4] ZHU Cai-hong, LI Shui-gen, QI Li-wang, HAN Su-ying. Agrobacterium tumefaciens-mediated Transformation of Larix leptolepis Embryogenic Tissue[J]. China Biotechnology, 2013, 33(5): 75-80.
[5] LI Shu-jie, LI Jin-wen, ZHANG Zheng-ying. Transformation and Identification of pta Gene into Wheat Cultivar Longchun22 Mediated by Agrobacterium tumefaciens[J]. China Biotechnology, 2012, 32(02): 50-56.
[6] LI Ming, LIU Meng, HUANG Yun-yan, ZHOU Li-ying, SUN Xin, LU Fu-ping. Establishment and Optimization of Agrobacterium tumefaciens-mediated Transformation System of Aspergillus niger[J]. China Biotechnology, 2012, 32(01): 56-63.
[7] ZOU Zhi. Advances on Factors Influencing Induction of Agrobacterium tumefaciens Virulence Genes[J]. China Biotechnology, 2011, 31(7): 126-132.
[8] . Agrobacterium Tumefaciens-mediated Transformation of Trichoderma atroviride for Improvement of Laccase Activity[J]. China Biotechnology, 2010, 30(07): 0-0.
[9] CI Mei-Ru, SU Chao, YANG Ge. Isolation and Identification of a High-efficiency Dye-degrading Bacteria Strain Xsmr and Its Degradation Characteristics[J]. China Biotechnology, 2010, 30(06): 70-76.
[10] . Advances in Biodegradation of Naphthalene[J]. China Biotechnology, 2009, 29(09): 0-0.
[11] HUANG Ya-li Xi-liang JIANG Yun-long TIAN . T-DNA insertional mutation of Trichoderma harzianum mediated by Agrobacterium tumefaciens and characteristic analysis of the transformants[J]. China Biotechnology, 2008, 28(3): 38-43.
[12] . Genetic Transformation of Phytoene Synthase Gene into Ginseng Callus Cells[J]. China Biotechnology, 2008, 28(2): 90-95.
[13] . Rice straw degradation with white rot fungi and cellulose multienzyme produced by Aspergillus Niger[J]. China Biotechnology, 2007, 27(3): 71-75.
[14] . Study on the Characteristics of Nicotine Degradation by Strain DN2 and Its Application[J]. China Biotechnology, 2006, 26(03): 47-50.
[15] . [J]. China Biotechnology, 1999, 19(3): 7-11.