中国生物工程杂志

Objective: To obtain a targeting cationic polymers (KH)20-EGF and examine its capacity for DNA condensation. Method: Prokaryotic expresssion plasmid pET28a-(KH)20-EGF was constructed and transformed into BL21(DE3). The cationic polypeptide was induced by IPTG and purified using a Ni-NTA column. The purified polypeptide was determined by SDS-PAGE and Western blot analysis. The pDNA package ability of the resulted protein was examined by gel retardation assay. Results: The yield of (KH)20-EGF protein was about 300μg/L. The resulted protein was a little larger than expected when examined by SDS-PAGE and Western blot. Gel retardation assays indicated that (KH)20-EGF can retard the pDNA migration. Conclusion: We successfully expressed the non-virus vector (KH)20-EGF and confirmed its capability of DNA condensation.