中国生物工程杂志

The feasibility of embryonic stem cells to combine with silk fibroin for neural differentiation was invaluable and unknown. We suspended the ES cells into EBs and then transferred them to three different substrates-coated 35 mm dishes including gelatin, Bombyx mori silk fibroin (SF) and Tussah silk fibroin (TSF) to identify the adherence and proportion of ES cells-derived neurons under these three substrates. The results showed that silk fibroin as a reliable biomaterial which can support EBs’ adherence and differentiated into euron-like cells compared with control group, all the proportion of β-Ⅲ-Tubulin positive cells is approximately 40%. The results further proved that the adhesion ability of cells on SF is poorer than on the control group and TSF group, which also showed that TSF is an excellent biomaterial for the neural differentiation of ES cells. This study may provide important experimental information for tissue engineering, in which ES cells-derived neuron cells and silk fibroin materials are scaffolds, and also offer a source for cell therapy research of neurodegenerative disease.