|
|
The empirical study of indoleamine 2,3-dioxygenase in contributing to the immune escape on hepatoma carcinoma cell |
|
|
Abstract This study was aimed to investigate the mechanism of indoleamine 2,3-dioxygenase(IDO) in HepG2 cells contributing to tumor immune escape. Methods T-lymphocyte and HepG2 cell were cocultured, and 1-methyl-tryptophan(1-MT) was added to make an intervening experiment. The expression of IDO mRNA in HepG2 cell cocultured with T-lymphocyte was detected by RT-PCR ;the apoptosis rate of HepG2 cell cocultured with T-lymphocyte was analyzed by flow cytometer; The cytotoxicity of T-lymphocyte against HepG2 cell was examined by MTT assay. Results In combine reaction system , the expression of IDO mRNA was strongly induced in HepG2 cell cocultured with T-lymphocyte and faintly induced when cultured with 1-MT ; the earlier apoptosis rate of HepG2 cell in control group and experiment group(1-MT:1.25 mmol/l,2.5 mmol/l,5mmol/l) was respectively(3.48±0.34)%,(7.82±0.41)%,(18.62±0.42)%,(25.32±0.40)%(P<0.01),The cytotoxicity of T-lymphocyte against HepG2 cell was respectively(17.36±1.24)%,(25.48±1.48)%,(32.89±1.73)%,(42.04±2.16)%(P<0.01). Conclusion These results suggest that IDO from HepG2 cell can suppress the tumor immunity of T-lymphocyte to kill hepatoma carcinoma cell, which may be contributing to hepatoma immune escape
|
Received: 29 December 2007
Published: 25 May 2008
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|