Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2008, Vol. 28 Issue (1): 119-123    DOI:
    
Novel progress in separation and purification technology of nattokinase
Download: HTML   PDF(613KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

The progress in separation and purification technology of nattokinase is reviewed. Based on the analyze of normal purification technology, the novel technologies about the integration of bioseparation processes are introduced, such as aqueous two-phase partitioning integrated with affinity ligand, expanded bed adsorption, and mixed-mode adsorption technology. In general, integration of bioseparation processes provides a good way to improve and optimize the whole separation process of nattokinase and other proteins.



Key wordsnattokinase      purification      integration      salt-independent adsorption     
Received: 24 July 2007      Published: 25 January 2008
Cite this article:

. Novel progress in separation and purification technology of nattokinase. China Biotechnology, 2008, 28(1): 119-123.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2008/V28/I1/119

[1] ZHANG Ling,CAO Xiao-dan,YANG Hai-xu,LI Wen-lei. The Application of Continuous Purification in Affinity Chromatography and Evaluation of Production Scale-up[J]. China Biotechnology, 2021, 41(6): 38-44.
[2] LV Yi-fan,LI Geng-dong,XUE Nan,LV Guo-liang,SHI Shao-hui,WANG Chun-sheng. Prokaryotic Expression, Purification of LbCpf1 Protein Gene and in Vitro Cleavage Activity Assay[J]. China Biotechnology, 2020, 40(8): 41-48.
[3] JIANG Dan-dan,WANG Yun-long,LI Yu-lin,Zhang Yi-qing. Study on the Delivery of RGD Modified Virus-Like Particles to ICG Targeted Tumors[J]. China Biotechnology, 2020, 40(7): 22-29.
[4] XIE Hang-hang,BAI Hong-mei,YE Chao,CHEN Yong-jun,YUAN Ming-cui,MA Yan-bing. The Purification Procedure for the Recombinant HBcAg Virus-like Particle Easy to Generate Aggregation[J]. China Biotechnology, 2020, 40(5): 40-47.
[5] WEI Wei,CHANG Bao-gen,WANG Ying,LU Fu-ping,LIU Fu-feng. Heterologous Expression, Purification and Aggregation Characterization of Tau Core Fragment 306-378[J]. China Biotechnology, 2020, 40(5): 22-29.
[6] LIU Zhen-zhen,TIAN Da-yong. Development of Sucrose Density Gradient Centrifugation Purification Process for Rabies Vaccine[J]. China Biotechnology, 2020, 40(4): 25-33.
[7] ZHU Tong-tong,YANG Lei,LIU Ying-bao,SUN Wen-xiu,ZHANG Xiu-guo. Purification and Crystallization of PcCRN20-C from Phytophthora capsici[J]. China Biotechnology, 2020, 40(1-2): 116-123.
[8] PAN Bing-jv,ZHANG Wan-yi,SHEN Hui-tao,LIU Ting-ting,LI Zhong-yuan,LUO Xue-gang,SONG Ya-jian. Research Progress on Separation and Purification of Mannan Oligosaccharide[J]. China Biotechnology, 2020, 40(11): 90-95.
[9] Yu-feng XIE,Xue-mei HAN,Fu-ping LU. Expression, Purification and Enzymatic Properties of β-glucosidase from Lactobacillus paracasei[J]. China Biotechnology, 2019, 39(5): 72-79.
[10] Song-tao ZHOU,Yun CHEN,Xiao-hai GONG,Jian JIN,Hua-zhong LI. Using CRISPR/Cas9 Technology to Construct Human Serum Albumin CHO Stable Expression Cell Line[J]. China Biotechnology, 2019, 39(4): 52-59.
[11] JING Jia-mei,XUN Xin,WANG Min,PENG Ru-chao,SHI Yi. Expression and Purification of C-terminal of Arenavirus Polymerase and Screening of Crystallization Conditions[J]. China Biotechnology, 2019, 39(12): 18-23.
[12] ZHU Meng-lu,WANG Xue-yu,LIU Xin,LU Fu-ping,SUN Deng-yue,QIN Hui-min. Heterologous Expression, Purification and Enzymatic Properties of a Novel Leucine 5-Hydroxylase[J]. China Biotechnology, 2019, 39(12): 24-34.
[13] Chao-di TONG,Jian-ping WU,Li-rong YANG,Gang XU. Crystal Structural Analysis of DehDIV-R by X-ray Crystallography[J]. China Biotechnology, 2018, 38(8): 19-25.
[14] Jun-jun CHEN,Ying LOU,Yuan-xing ZHANG,Qin LIU,Xiao-hong LIU. Expression and Purification of Proliferating Cell Nuclear Antigen in Spodoptera frugiperda Cells[J]. China Biotechnology, 2018, 38(7): 14-20.
[15] Shi-jie LI,Yan-kun YANG,Meng LIU,Zhong-hu BAI,Jian JIN. Efficient Expression of SUMO Protease Ulp1 and Used to Express and Purified scFv by His-SUMO tag[J]. China Biotechnology, 2018, 38(3): 51-61.