Proteomics Analysis of heteromorphic leaves of Populus euphratica Oliv

China Biotechnology

2009, Vol. 29

Issue (09): 0-0 DOI:

Proteomics Analysis of heteromorphic leaves of Populus euphratica Oliv

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Abstract By exploring the different components of the lysis buffer and optimize the 2-DE conditions, established the best proteomics technical system for Populus euphratica’s heteromorphic leaves, while take the heteromorphic leaves in the same blanche as the test materials to find differences between the protein expressions of the leaves. It showed that the lysis solution which containing 2mmol/L thiourea,7mmol/L urea, 2% CHAPS,60mmol/L DTT and 0.2% IPG buffer could dissolve the protein better. Through tandem mass spectrum, the results show that heteromorphic leaves are different in photosynthesis and respiration. This research offered valuable informations for understanding the molecular mechanism during leaves development and elucidating the mechanism of the eco-adaptability of Populus euphratica.

Key words： Populus euphratica Oliv Heteromorphic leaves Proteomics

Received: 28 April 2009 Published: 10 May 2010

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	YUE Ning
	ZHENG Cai-Xia
	BO Xue
	HAO Jian-Qing

Cite this article:

. Proteomics Analysis of heteromorphic leaves of Populus euphratica Oliv. China Biotechnology, 2009, 29(09): 0-0.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I09/0

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