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| Studies on Dot-ELISA Method for Detecting GPV Infected Antibody |
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Abstract The method of Dot-ELISA for detecting GPV antibody was established with recombinant prokaryotic expressed peptide of VP1-VP3 non-repeated nucleotide sequences of GPV capsid protein in this test. The coating concentration of Dot-ELISA detecting antigen was 700ng. The optimum dilution of rabbit anti-goose IgG-HRP antibody and detected serum were 1:200 and 1:400 respectively. The positive rate of detected GPV serum antibody was 100%, and the other one of the chicken anti-GPV-VP3 gene recombinant poutry poxvirus serum antibody was 0%.
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Received: 02 December 2008
Published: 10 May 2010
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Corresponding Authors:
（中）布日额 （英）BU Ri-e
E-mail: wjhbre@yahoo.com.cn
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