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| Cloning and Identification of the Second Intron of an AGAMOUS-like Gene from Peach (Prunus persica) |
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Abstract In this study the second intron, pPpMADS4, of an AGMOUS (AG) homologous gene PpMADS4 was cloned from peach (Prunus persica) and its function studied in transgenic Arabidopsis thaliana. Sequence analysis revealed the presence of several putative cis elements in the second intron of PpMADS4, and all the second introns cloned from seven different peach cultivars possess highly conserved cis elements although SNP-rich regions were identified. Furthermore, the pPpMADS4∷minimal35S∷GUS-0029 vector were constructed and transformed into wild-type A. thaliana. β-glucoronidase staining showed that it was expressed specifically in carpel and stamen at late floral developmental stage.
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Received: 29 April 2008
Published: 25 September 2008
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