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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (1): 14-22    DOI: 10.13523/j.cb.20160103
    
Effect of Exogenous S100A6 Protein on The Level of S100A6 mRNA of Colorectal Carcinoma Cell Lines and Its Possible Mechanism
SUN Hui, LI Xue-ru, ZHA He, DUAN Liang, YUAN Shi-mei, LI Huan, LI Ai-fang, GU Yue, ZHOU Lan
Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
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Abstract  

Objective: To investigate the effect of rhS100A6 on S100A6 mRNA of colorectal carcinoma cell lines HCT116 and SW480, and to explore its possible mechanism. Methods: Recombinant human S100A6 (rhS100A6) protien was prepared by prokaryotic expression. FHC, HCT116 and SW480 cells were treated with 10μg/ml rhS100A6 and recombinant adenoviruses carrying human β-catenin siRNA (Adsiβ-catenin), semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real time polymerase chain reaction (qPCR) was used to detect the expression of S100A6 mRNA, RT-PCR was used to detect the expression of E-cadherin mRNA,Western blot was used to detect the expression of β-catenin of nuclear protein in HCT116 and SW480 cells and immunofluorescence staining was used to detect the distribution of β-catenin. Results: rhS100A6 protein was prepared successfully and significantly increased the expression of S100A6 mRNA and nuclear β-catenin protein of HCT116 and SW480 cells, rhS100A6 treatment promoted β-catenin translocating from cytoplasm to nucleus in HCT116 and SW480 cells, at the same time, the E-cadherin mRNA level was decreased with the treatment of rhS100A6 protein. Compared with rhS100A6 treatment group, the expression level of S100A6 mRNA was decreased in co-treatment group by Adsiβ-catenin and rhS100A6. Conclusion: Extracellular rhS100A6 upregulate the experssion of S100A6 mRNA in colorectal carcinoma cell lines and its mechanism is partly involved in Wnt/β-catenin pathway.



Key wordsS100A6      Colorectal carcinoma      β-catenin     
Received: 20 July 2015      Published: 11 January 2016
ZTFLH:  Q819  
Cite this article:

SUN Hui, LI Xue-ru, ZHA He, DUAN Liang, YUAN Shi-mei, LI Huan, LI Ai-fang, GU Yue, ZHOU Lan. Effect of Exogenous S100A6 Protein on The Level of S100A6 mRNA of Colorectal Carcinoma Cell Lines and Its Possible Mechanism. China Biotechnology, 2016, 36(1): 14-22.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160103     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I1/14

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