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| Research Progress on Aminoacyl-tRNA Synthetases |
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Abstract Aminoacyl-tRNA synthetases catalyze the attachment of specific amino acids to their cognate tRNAs, thereby ensuring the faithful translation of genetic code. In addition to their enzymatic function, these ancient and conserved enzymes have been discovered to have many additional functions in mammalian cells. As a result, several application aspects have been found. Aminoacyl-tRNA synthetases have attracted interest as essential and novel targets involved in bacterial protein synthesis, and they are now being pursued as targets for new antibiotic drugs. Engineered aminoacyl-tRNA synthetases are uesed for site-specifically incorporation of non-natural amino acids. The results with animals suggest that therapeutic applications for many human diseases such as tumor are possible with the cognate tRNA synthetases.
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Received: 22 August 2008
Published: 02 July 2009
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Corresponding Authors:
ZHOU Qin-hua
E-mail: qinhuazhou_0522@163.com
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| ［1］ Hausmann C D, Ibba M. AminoacyltRNA synthetase complexes: molecular multitasking revealed. FEMS Microbiol Rev,2008,32(4): 705~721
［2］ Han J M, Kim J Y, Kim S. Molecular network and functional implications of macromolecular tRNA synthetase complex. Biochem Biophys Res Commun, 2003,303(4):985~993
［3］ Greenberg Y, King M, Kiosses W B, et al. The novel fragment of tyrosyl tRNA synthetase, miniTyrRS, is secreted to induce an angiogenic response in endothelial cells. FASEB J, 2008,22(5):1597~1605
［4］ Pohlmann J, BrtzOesterhelt H. New aminoacyltRNA synthetase inhibitors as antibacterial agents. Curr Drug Targets Infect Disord, 2004,4(4):261~272
［5］ Kim S, Lee S W, Choi E C, et al. AminoacyltRNA synthetases and their inhibitors as a novel family of antibiotics. Appl Microbiol Biotechnol, 2003,61(4):278~288
［6］ Mukai T, Kobayashi T, Hino N, et al. Adding llysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyltRNA synthetases. Biochem Biophys Res Commun, 2008,371(4):818~822
［7］ Kiga D, Sakamoto K, Kodama K, et al. An engineered Escherichia coli tyrosyltRNA synthetase for sitespecific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cellfree system. Proc Natl Acad Sci USA, 2002,99(15):9715~9720
［8］ Kobayashi T, Sakamoto K, Takimura T, et al. Structural basis of nonnatural amino acid recognition by an engineered aminoacyltRNA synthetase for genetic code expansion. Proc Natl Acad Sci USA, 2005,102(5):1366~1371
［9］ Ruan B, Sll D. The bacterial YbaK protein is a CystRNAPro and CystRNA Cys deacylase. J Biol Chem,2005,280 (27):25887~25891
［10］ Bailly M, Blaise M, Lorber B, et al. The transamidosome: a dynamic ribonucleoprotein particle dedicated to prokaryotic tRNAdependent asparagine biosynthesis. Mol Cel ,2007,28(2):228~239
［11］ PraetoriusIbba M, Rogers T E, Samson R, et al. Association between Archaeal prolyl and leucyltRNA synthetases enhances tRNA(Pro) aminoacylation. J Biol Chem, 2005,28 0(28):26099~26104
［12］ Karanasios E, Simader H, Panayotou G, et al. Molecular determinants of the yeast Arc1paminoacyltRNA synthetase complex assembly. J Mol Biol,2007,374(4):1077~1090
［13］ Guzzo C M, Yang D C. LysyltRNA synthetase interacts with EF1alpha, aspartyltRNA synthetase and p38 in vitro. Biochem Biophys Res Commun,2008,365(4):718~723
［14］ 徐敏刚. 亮氨酰tRNA 合成酶和 tRNALeu的相互作用.上海:中国科学院上海生命科学研究院,2004.
Xu M G. The interaction between leucyltRNA synthetase and tRNALeu. Shanghai: Shanghai Institutes for Biological Sciences,Chinese Academy of Science, 2004
［15］ 刘云清. 大肠杆菌亮氨酰tRNA合成酶编校结构域和其与蛋氨酸和异亮氨酸复合物晶体结构的研究. 上海:中国科学院上海生命科学研究院,2006
Liu Y Q. The crystal structures of the editing domain of E. coli leucyltRNA synthetase and its complexes with methionine and isoleucine. Shanghai: Shanghai Institutes for Biological Sciences,Chinese Academy of Science, 2006
［16］ Kowal A K, Kohrer C, RajBhandary U L. Twentyfirst aminoacyltRNA synthetasesuppressor tRNA pairs for possible use in sitespecific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria. Proc Natl Acad Sci USA, 2001,98(5):2268~2273
［17］ Tang Y, Tirrell D A. Attenuation of the editing activity of the Escherichia coli leucyltRNA synthetase allows incorporation of novel amino acids into proteins in vivo. Biochemistry, 2002 ,41(34):10635~10645
［18］ Kiick K L, Weberskirch R, Tirrell D A. Identification of an expanded set of translationally active methionine analogues in Escherichia coli. FEBS Lett, 2001,502(12):25~30
［19］ Rock F L, Mao W, Yaremchuk A, et al. An antifungal agent inhibits an aminoacyltRNA synthetase by trapping tRNA in the editing site. Science,2007,316(5832):1759~1761
［20］ Bernier S, Akochy P M, Lapointe J, et al. Synthesis and aminoacyltRNA synthetase inhibitory activity of aspartyl adenylate analogs. Bioorg Med Chem,2005,13(1):69~75
［21］ Balg C, Blais S P, Bernier S, et al. Synthesis of betaketophosphonate analogs of glutamyl and glutaminyl adenylate, and selective inhibition of the corresponding bacterial aminoacyltRNA synthetases. Bioorg Med Chem, 2007,15(1):295~304
［22］ Otani A, Slike B M, Dorrell M I, et al. A fragment of human TrpRS as a potent antagonist of ocular angiogenesis. Proc Natl Acad Sci USA,2002,99(1) : 178~183
［23］ Tzima E, Schimmel P. Inhibition of tumor angiogenesis by a natural fragment of a tRNA synthetase. Trends Biochem Sci, 2006 ,31(1):7~10
［24］ Edvardson S, Shaag A, Kolesnikova O, et al. Deleterious mutation in the mitochondrial arginyltransfer RNA synthetase gene is associated with pontocerebellar hypoplasia. Am J Hum Genet, 2007,81(4): 857~862
［25］ Scheper G C, van der Klok T, van Andel R J, et al. Mitochondrial aspartyltRNA synthetase deficiency causes leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation.Nat Genet, 2007, 39 (4): 534~539
［26］ 't Hart L M, Hansen T, Rietveld I, et al. Evidence that the mitochondrial leucyl tRNA synthetase (LARS2) gene represents a novel type 2 diabetes susceptibility gene. Diabetes, 2005, 54 (6):1892~1895
［27］ 朱斌,王恩多. 氨基酰tRNA 合成酶与神经退行性疾病. 生物化学与生物物理进展, 2007, 34(6): 562~566
Zhu B,Wang E D. Neurodegenerationrelated AminoacyltRNA Synthetases. Progress in Biochemistry and Biophysics, 2007, 34(6): 562~566
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