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| Effect of Osmotic Pressure on Glycerol Production and Intracellular Phosphorus Accumulation by Candida glycerinogenes |
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Abstract Effects of osmotic pressure on glycerol production and intracellular phosphorus accumulation by Candida glycerinogenes at different phosphate concentrations was studied. The results showed that, at different KH2PO4 concentrations, the more yield of glycerol synthesis by C. glycerinogenes, the higher yields of extracellular and intracellular glycerol. There is a suitable osmotolerance that the maximum glycerol production is achieved by C.glycerinogenes at the same KH2PO4 concentration. Similarly, there is a suitable phosphorus concentration to produce the maximum glycerol yields at the same osmotolerance. The increase in osmotic pressure can promote the accumulation of intracellular polyphosphate (PolyP) at the same KH2PO4 concentration. So the increase in KH2PO4 concentration does at the same osmotic pressure. At the late of stationary phase, superfluous phosphorus can clearly enhance the accumulation of intracellular free phosphorus and PolyP. The analysis demonstrated that the accumulation of intracellular glycerol and PolyP could play an important role in overcoming the trouble of less cells and large osmotic pressure, thus maintaining its larger biomass concentration, cell survival and glycerol production.
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Received: 20 November 2008
Published: 27 April 2009
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