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| Optimization of Genetic Transformation System of Sugarcane Callus Mediated by Agrobacterium |
| QIN Cui-xian1,2, CHEN Zhong-liang1,2, GUI Yi-yun1,2, WANG Miao1,2, ZHOU Jian-hui2,3, LIAO Qing2,3, LI Yang-rui1,2, HUANG Dong-liang1,2 |
1. Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, P.R.China, Nanning 530007, China;
2. Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China;
3. Guangxi Academy of Agricultural Sciences, Nanning 530007, China |
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Abstract To establish high-efficiency sugarcane transformation system, the optimal concentration of antibiotic (Km) was screened for subculture and differentiation of sugarcane calli, as well as seedling rooting, respectively. The factors that influenced sugarcane transformation, such as pre-culture time, infection time and co-culture time were also studied. The results showed that the optimal concentrations of antibiotic (Km) for subculture, differentiation and seedling rooting were 300 mg/L, 40 mg/L and 20 mg/L, respectively. The optimal pre-culture time, infection time and co-culture time were 4d, 30min and 4d, respectively. PCR detection showed that 61.6% of resistance plants were transformed plants. This work lays a solid foundation for further study on gene function and transgenic research of sugarcane.
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Received: 19 June 2013
Published: 25 September 2013
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Cite this article:
QIN Cui-xian, CHEN Zhong-liang, GUI Yi-yun, WANG Miao, ZHOU Jian-hui, LIAO Qing, LI Yang-rui, HUANG Dong-liang,. Optimization of Genetic Transformation System of Sugarcane Callus Mediated by Agrobacterium. China Biotechnology, 2013, 33(9): 66-72.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2013/V33/I9/66
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