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| Research Progress of Genome Editing in Plants |
| XIE Ke1,2, RAO Li-qun3, LI Hong-wei1,2, AN Xue-li1,2, FANG Cai-chen1,2,4, WAN Xiang-yuan1,2,3,4 |
1. State Key Laboratory of Main Crop Germplasm Innovation, Beijing 100192, China;
2. Beijing Golden Guanfeng Bio-tech Co., LTD, Beijing 100192, China;
3. Hunan Agricultural University, College of Bioscience and Biotechnology, Changsha 410128, China;
4. Shandong Guanfeng Seed Science and Technology Co., LTD, Guanxian 252500, China |
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Abstract The precise insertion of a foreign DNA molecule at genome through homologous recombination remains low efficiency in plants. Genome editing is an important tool to precisely integrate DNA molecules at a defined genomic location. Extensive efforts have been made to understand the mechanisms governing gene targeting and to establish efficient systems to achieve precise and efficient targeting. A set of genome editing techniques, engineered meganucleases, zinc finger nucleases, and transcription activator-like effector nucleases, have recently emerged that enable targeted editing of genomes in plants. The recent development of genome editing technique based on the CRISPR/Cas system demonstrate that it is efficient and specific for wide application. The rapid progress in the field of genome editing was summarized, and then the potential perspective of the genome editing technology to be used in agriculture and plant engineering was discussed.
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Received: 02 May 2013
Published: 25 June 2013
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