The Overexpression of PHP14 and Its Effect on NIH-3T3 Cells Proliferation <em>in vitro</em> and Anchor Independent Growth

China Biotechnology

2013, Vol. 33

Issue (6): 7-11 DOI:

The Overexpression of PHP14 and Its Effect on NIH-3T3 Cells Proliferation in vitro and Anchor Independent Growth

XU An-jian^1,2, CUI Yong³, ZHAO Gao-chao², WU Shan-na², LÜ Zhi⁴, JIN Qing-e², GU Jun-chao¹

1. Beijing Friendship Hospital, Beijing 100050, China;
2. Viral Laboratory, Beijing Friendship Hospital, Beijing 100050, China;
3. Department of Chest Surgery, Beijing Friendship Hospital, Beijing 100050, China;
4. Department of Clinical Laboratories, Beijing Friendship Hospital, Beijing 100050, China

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Abstract

Objective: To amplify human PHP14 gene, and construct the N-terminal and C-terminal GFP fused vectors, establish the overexpress PHP14 NIH-3T3 cell line to investigate its effect on NIH-3T3 cell proliferation and anchor independent growth. Methods: The cDNA sequence of PHP14 gene was amplified and sub-cloned into pEGFP-N and pEGFP-C3 vectors. Transfecting of those vectors into NIH-3T3 cells and investigating the cell proliferation and anchor independent growth in PHP14 overexpressed NIH-3T3 cells using MTT and Soft agar colony assay. Results: The pEGFP-N2-PHP14 and pEGFP-C3-PHP14 prokaryotic expression vectors were constructed successfully and the expression of those vectors were detected in NIH-3T3 cells. Overexpression of PHP14 in NIH-3T3 cells did not affect the cell proliferation of NIH-3T3 cells, but gave the NIH-3T3 cells the ability of anchor independent growth. Conclusion: PHP14 prokaryotic expression vectors were constructed successfully and overexpression of PHP14 in NIH-3T3 cells did not affect the cell proliferation of NIH-3T3 cells, but gave the NIH-3T3 cells the ability of anchor independent growth.

Key words： PHP14 Overexpression Cell proliferation Anchor independent growth

Received: 15 February 2013 Published: 25 June 2013

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	XU An-jian
	CUI Yong
	ZHAO Gao-chao
	WU Shan-na
	LÜ Zhi
	JIN Qing-e
	GU Jun-chao

Cite this article:

XU An-jian, CUI Yong, ZHAO Gao-chao, WU Shan-na, LÜ Zhi, JIN Qing-e, GU Jun-chao. The Overexpression of PHP14 and Its Effect on NIH-3T3 Cells Proliferation in vitro and Anchor Independent Growth. China Biotechnology, 2013, 33(6): 7-11.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2013/V33/I6/7

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