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Spongelike Small Intestinal Submucosa Matrix Can Promote Osteoblast-like Cells Proliferation and Differentiation |
FANG Yan1, NI Wei-min2, SHAN Wei1, ZENG Rui-xia1, LIU Xue-yuan1 |
1. Department of Anatomy of Basic Medical College of LiaoNing Medical University, Jinzhou 121000, China;
2. Department of Neurosurgery of the First Affiliated Hospital of LiaoNing Medical University, Jinzhou 121000, China |
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Abstract Objective: Observe the growth of osteoblast-like cells induced and differentiated by human adipose stem cells (hADSCs) in the spongelike porcine small intestinal matrix (SIS). Explore ability of three-dimensional SIS promote osteoblast-like cells proliferation and differentiation. Methods: acellular SIS is prepared by combination of physical and chemical methods with porcine proximal jejunum. Particles was made with film-like SIS by milling-in liquid nitrogen cryogenic grinding. And the particles has been reshaped spongelike after crosslinked by freeze-drying technology. hADSCs is isolated and cultured by enzymatic digestion. And surface antigen has been identificated by flow cytometry. hADSCs has been induced and differentiated into osteoblast-like cells、chondroblast-like cells and adipocyte-like cells. The osteoblast-like cells is cultured in cavernous SIS. Cell morpholog is observed by scanning electron microscope. Osteoblast-like cells is cultured in material extracts of SIS. The cell viability is evaluated with MTT. Osteogenic differentiation is detected by ALP activity. Results: Spongelike SIS is three-dimensional stereo-scaffold with a great of regular three-dimensional pore. Stem cell-associated antigen is expressed by primary hADSCs. hADSCs can differentiated into osteoblast-like cells stained by alizarin red. Osteoblast-like cells can proliferate and express ALP obviously in cavernous SIS. Conclusion: spongelike SIS with three-dimensional pores presents superior cytocompatibility, and it can promote osteoblast-like cell differentiated by hADSCs proliferate and increase osteogenic activity significantly. It can became a novel three-dimensional natural biological materials for bone tissue engineering.
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Received: 29 January 2013
Published: 25 June 2013
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