Construction of Co-expression Vector Containing AtCAO and AtHEMA1 Genes from Arabidopsis and Transformation into Tobacco

China Biotechnology

2013, Vol. 33

Issue (4): 54-60 DOI:

Construction of Co-expression Vector Containing AtCAO and AtHEMA1 Genes from Arabidopsis and Transformation into Tobacco

LI Cui-ping, PAN Yu, BAI Xiao-ning, CHU Fu-tang, SU Cheng-gang, ZHANG Xing-guo

Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Key College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China

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Abstract It is a possible way to improve the low light resistance of crops by increasing the amount of chlorophyll, especially that of chlorophyll b. AtHEMA1 and AtCAO from Arabidopsis thaliana were constructed into the binary expression vector pCAMBIA1302. This two genes were introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. Both the amount of chlorophyll b and the rate of photosynthesis were remarkably increased in the transgenic plant lines TL1 and TL2 compared with the wild type tobacco, while the chlorophyll a/b decreased significantly(P<0.01). The growth of the transgenic tobacco was accelerated compared with the WT under low light. The results had laid a foundation for improving crop low light tolerance with those two genes.

Key words： Tobacco AtHEMA1 gene AtCAO gene Chlorophyll Low light resistance

Received: 18 January 2013 Published: 25 April 2013

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	LI Cui-ping
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	ZHANG Xing-guo

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LI Cui-ping, PAN Yu, BAI Xiao-ning, CHU Fu-tang, SU Cheng-gang, ZHANG Xing-guo. Construction of Co-expression Vector Containing AtCAO and AtHEMA1 Genes from Arabidopsis and Transformation into Tobacco. China Biotechnology, 2013, 33(4): 54-60.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2013/V33/I4/54

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